Web server specs: Windows 2008 R2 server 64-bit;
Website built with SharePoint 2007 64 bit;
The error: eo_web.ashx does not exist at the location specified by configuration setting eo_HandlerLocation is displayed when the Master page quite a while to render or the home page to come up as a possibility.
Has anyone else reported this error and was there a fix.
The entry in the web.config file:
 <add key="eo_HandlerLocation" value="~/">
    </add>
the EO.web.dll is located in the bin folder of the website
the eo_web.ashx is in the root folder of the website where the web.config file is.
The IIS website has .Net trust level set to Full (Internal).
Log Name:      Application
Source:        ASP.NET 2.0.50727.0
Date:          29/10/2013 12:25:50 PM
Event ID:      1309
Task Category: Web Event
Level:         Warning
Keywords:      Classic
User:          N/A
Computer:      PER-SPWFE.nntt.gov.au
Description:
Event code: 3005 
Event message: An unhandled exception has occurred. 
Event time: 29/10/2013 12:25:50 PM 
Event time (UTC): 29/10/2013 4:25:50 AM 
Event ID: 123adc22e3844b759c7fec1379d3abdc 
Event sequence: 15 
Event occurrence: 1 
Event detail code: 0  
Application information: 
    Application domain: /LM/W3SVC/442333377/ROOT-1-130274943410118221 
    Trust level: Full 
    Application Virtual Path: / 
    Application Path: C:\inetpub\wwwroot\wss\VirtualDirectories\www.nntt.gov.au80\ 
    Machine name: PER-SPWFE  
Process information: 
    Process ID: 3904 
    Process name: w3wp.exe 
    Account name: xxxxxx 
Exception information: 
    Exception type: HttpException 
    Exception message: Virtual Url 9cc2706e-16d5-4bcf-a16b-179addb58c8e not registered.  
Request information: 
    Request URL: 
http://www.nntt.gov.au/eo_web.ashx?id=9CC2706E-16D5-4bcf-A16B-179ADDB58C8E     Request path: /eo_web.ashx 
    User host address: xxxxxxxx
    User:  
    Is authenticated: False 
    Authentication Type:  
    Thread account name: xxxxxx 
Thread information: 
    Thread ID: 12 
    Thread account name: xxxxxx
    Is impersonating: True 
    Stack trace:    at EO.Web.Internal.cr.a(cl A_0)
   at EO.Web.Internal.ds.a(HttpContext A_0)
   at EO.Web.Runtime.ProcessRequest(HttpContext context)
   at System.Web.HttpApplication.CallHandlerExecutionStep.System.Web.HttpApplication.IExecutionStep.Execute()
   at System.Web.HttpApplication.ExecuteStep(IExecutionStep step, Boolean& completedSynchronously)  
Custom event details: 
Event Xml:
<Event xmlns="http://schemas.microsoft.com/win/2004/08/events/event">
  <System>
    <Provider Name="ASP.NET 2.0.50727.0" />
    <EventID Qualifiers="32768">1309</EventID>
    <Level>3</Level>
    <Task>3</Task>
    <Keywords>0x80000000000000</Keywords>
    <TimeCreated SystemTime="2013-10-29T04:25:50.000000000Z" />
    <EventRecordID>6072</EventRecordID>
    <Channel>Application</Channel>
    <Computer>PER-SPWFE.nntt.gov.au</Computer>
    <Security />
  </System>
  <EventData>
    <Data>3005</Data>
    <Data>An unhandled exception has occurred.</Data>
    <Data>29/10/2013 12:25:50 PM</Data>
    <Data>29/10/2013 4:25:50 AM</Data>
    <Data>123adc22e3844b759c7fec1379d3abdc</Data>
    <Data>15</Data>
    <Data>1</Data>
    <Data>0</Data>
    <Data>/LM/W3SVC/442333377/ROOT-1-130274943410118221</Data>
    <Data>Full</Data>
    <Data>/</Data>
    <Data>C:\inetpub\wwwroot\wss\VirtualDirectories\www.nntt.gov.au80\</Data>
    <Data>PER-SPWFE</Data>
    <Data>
    </Data>
    <Data>3904</Data>
    <Data>w3wp.exe</Data>
    <Data>NNTT1\sp_admin</Data>
    <Data>HttpException</Data>
    <Data>Virtual Url 9cc2706e-16d5-4bcf-a16b-179addb58c8e not registered.</Data>
    <Data>http://www.nntt.gov.au/eo_web.ashx?id=9CC2706E-16D5-4bcf-A16B-179ADDB58C8E</Data>
    <Data>/eo_web.ashx</Data>
    <Data>10.10.0.24</Data>
    <Data>
    </Data>
    <Data>False</Data>
    <Data>
    </Data>
    <Data>useridxxxx</Data>
    <Data>12</Data>
    <Data>useridxxxx</Data>
    <Data>True</Data>
    <Data>   at EO.Web.Internal.cr.a(cl A_0)
   at EO.Web.Internal.ds.a(HttpContext A_0)
   at EO.Web.Runtime.ProcessRequest(HttpContext context)
   at System.Web.HttpApplication.CallHandlerExecutionStep.System.Web.HttpApplication.IExecutionStep.Execute()
   at System.Web.HttpApplication.ExecuteStep(IExecutionStep step, Boolean& completedSynchronously)
</Data>
  </EventData>
</Event>
Is there anything that I need to change or do?
Please help urgently with this matter?
Much appreciated.
Cheers
Darryl